The Asian longhorned beetle, Anoplophora glabripennis, is an invasive pest that poses a serious threat to many species of North American hardwoods. An efficacious heat treatment schedule for this insect is crucial to allow wood to move from quarantined areas in the United States. A series of experiments were conducted using naturally infested trees to evaluate the International Standards for Phytosanitary Measures Rule No. 15 heat treatment schedule (56°C core temperature for 30 min) on overwintering A. glabripennis larvae. Results indicate that this treatment is effective, as no overwintering larvae were observed to survive. Overwintering larval stage, heating rates, oven load factors, and treatments are reported.